Heat and Acetic Acid Test

Planning/Implementation:

Gather equipment needed, as follows: test tube, 5% acetic acid, Fill a test tube ¾ full of clean urine and gently heat the upper portion to boil, boil for 1-2 minutes. A turbidity is either due to phosphates, carbonates or albumin. Add 3 drops of 5% acetic acid drop by drop, doiling between each drop. A white-cloud now appearing is due to early phosphate or carbonates, a faint trace of albumin may appear only  upon the addition of the acid. The addition of too much acid may dissolve faint traces of albumin and give a faculty negative reaction. In order to detest slight traces, the tube must be held against a black background. Record results as:

Negative -       no closeness is perceptible

Trace -             no cloudiness is perceptible against a black background

+          -           cloudiness is distinct but not gramular against a black background and can barely be seen when held up to the light.

+ + +   -           cloud is distinct and gramular light (0.2 – 0.5 Gm. %)

+ + + + + -       cloud is dense with large flocculi, any solidity ( 0.5 Gm. %) albumin becomes solid and boiling.