Planning/Implementation:
Gather equipment needed, as follows: test tube, 5% acetic acid, Fill a test tube ¾ full of clean urine and gently heat the upper portion to boil, boil for 1-2 minutes. A turbidity is either due to phosphates, carbonates or albumin. Add 3 drops of 5% acetic acid drop by drop, doiling between each drop. A white-cloud now appearing is due to early phosphate or carbonates, a faint trace of albumin may appear only upon the addition of the acid. The addition of too much acid may dissolve faint traces of albumin and give a faculty negative reaction. In order to detest slight traces, the tube must be held against a black background. Record results as:
Negative - no closeness is perceptible
Trace - no cloudiness is perceptible against a black background
+ - cloudiness is distinct but not gramular against a black background and can barely be seen when held up to the light.
+ + + - cloud is distinct and gramular light (0.2 – 0.5 Gm. %)
+ + + + + - cloud is dense with large flocculi, any solidity ( 0.5 Gm. %) albumin becomes solid and boiling.
what is the purpose of adding glacial acetic acid in the heat coagulation test?
first heating time turbidity is maybe albumin or phosphates present.
for find out albumin in adding acetic acid then heating.
Acetyl Tetrapeptide-11 is the reaction product of Acetic Acid and tetrapeptide-11, containing leucine, proline and tyrosine residues. It promotes the cell growth, Syndecan-1 and Collagen XVII synthesis.Acetyl Tetrapeptide-11